Size-based chromosome separation in a microfluidic particle separation device using viscoelastic fluids

Viscoelastic flow-based particle manipulation techniques enable bio-particle focusing, separation, and enrichment by precisely tuning the rheological parameters, flow conditions, microchannel geometry. In this study, we fabricated a PDMS-based single inlet/outlet to separate bio-particles their size ranging from 1-10 μm. Flow conditions properties are optimized using 2 μm 4 Polystyrene beads reach best separation condition. We demonstrated size-based of human chromosomes separating 1-2 small 8-10 large chromosomes. Thanks its miniaturized simplicity, isolation chip unique viscoelastic method have great potential be used as future pioneering tool for genetic applications study chromosome abnormalities such fragile-X trisomy.